🔲 Quantitative enzymatic degradation assays (e.g., PET nanoparticle breakdown by PETase)

🔲 Photodegradation and thermal degradation tests under simulated environmental conditions

Reference studies

1) Rapid depolymerization of poly(ethylene terephthalate) thin films by a dual-enzyme system and its impact on material properties (https://doi.org/10.1016/j.checat.2022.11.004)

2) Recent trends in degradation of microplastics in the environment: A state-of-the-art review

(https://doi.org/10.1016/j.hazadv.2023.100343)

🔲Detection and quantification of micro/nanoplastics in water, soil, and air

🔲 Calibration and validation standards for analytical instruments (e.g., Raman, FT-IR, NTA, DLS, SR-SIM, CLSM, etc.)

Reference studies

1) Micro- and nanoplastics in the environment: a comprehensive review on detection techniques (https://doi.org/10.1039/D4EN00319E)

2) Applications of Fluorescence Technology for Rapid Identification of Marine Plastic Pollution (https://doi.org/10.3390/polym17121679)

🔲 Uptake and accumulation of nanoplastics in aquatic organisms

🔲 Cellular uptake and cytotoxicity assays (e.g., ROS generation, inflammation, internalization)

Reference studies

1) Review of mechanisms and impacts of nanoplastic toxicity in aquatic organisms and potential impacts on human health (https://doi.org/10.1016/j.toxrep.2025.102013)

2) The Cytotoxicity of Biodegradable Microplastics and Nanoplastics: Current Status and Research Prospects (https://doi.org/10.3390/microplastics4030058)